Autoantibodies : Methods and Protocols /
edited by Gunnar Houen.
Bok Engelsk 2019 · Electronic books.
Medvirkende | Houen, Gunnar. (editor.)
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Omfang | 1 online resource (XII, 289 p. 58 illus., 32 illus. in color.)
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Utgave | 1st ed. 2019.
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Opplysninger | Autoantibodies as Diagnostic Tools -- Non-specific Binding in Immunoassays for Autoantibodies -- Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on HEp-2 Cells -- Detection of Anti-Neutrophil Cytoplasmic Antibodies by Indirect Immunofluorescence -- Determination of Subset-Restricted Anti-Neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry -- Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry -- Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry -- Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay -- Determination of Autoantibodies to Salivary Gland Antigens -- Measurement of autoantibodies to the gastric H+,K+-ATPase (ATP4A/B) using a Luciferase Immuno Precipitation System (LIPS) -- Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay -- Determination of Autoantibodies to Neutrophil Granule Constituents by Solid Phase Immunoassay -- Determination of Autoantibodies to C3 Nephritic Factors by ELISA -- Determination of Anti-C1q Autoantibodies by Western Blot -- Anti-Factor H Autoantibodies Assay by ELISA -- Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay -- Determination of CRP Autoantibodies by SPR Immunoassay -- Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis -- Detection of SSA and SSB Autoantibodies by Enzyme-Linked Immunosorbent Assay -- Assessment of Peptidylarginine Deiminase Activity by ELISA using Human Fibrinogen as Substrate -- The Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay -- Determination of Autoantibodies by Line Blotting -- Determination of Rheumatoid Factors by ELISA -- Detection of autoantibodies to complement components by surface plasmon resonance-based technology. .. - This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients.
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ISBN | 1-4939-8949-9
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