KP-1 is a marker for extraneuronal neurofibrillary tangles and senileplaques in Alzheimer diseased brains
K. Kobayashi
Bok Engelsk 1998
| Utgitt | 1998
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| Omfang | Side 13- 19
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| Opplysninger | KP-1 immunostaining with microwave pretreatment in formalin-fixed,paraffin-embedded sections enhanced its immunoreactivity revealingextraneuronal neurofibrillary tangles (NFTs) called ghost tangles,senile plaques (SPs) and perivascular deposits as well as microgliallabelling in Alzheimer-diseased brains. KP-1 stained cored anduncored SPs, granules within the SPs, perivascular beta-amyloidprotein (beta AP) and star-like beta AP deposits in cortical layer I,which was confirmed in comparison to silver-impregnated structures inthe Reusche-stained or Gallyas-Schiff-stained sections. On doubleimmunostaining with KP-1 and ubiquitin, ghost tangles were labelledby KP-1 and intraneuronal NFTs were positive for ubiquitin. A few KP-1-positive granules deposits different from amyloid core were foundwithin the SPs and the outer margin of amyloid cores of SPs werestained by KP-1. KP-1-positive microglia were attached to theubiquitin-positive intraneuronal NFTs. Microglia were more numerouslylabelled by CR3/43 than by KP-1, and CR3/43-positive microglia werefound to be preferentially attached to SPs. As KP-1 recognizeslysosome-associated antigen CD68, similarities between KP-1positivity and Reusche-stained structures suggested that lysosomalactivity was associated with beta AP deposits and ghost tangles wereinvolved in lysosome-associated processes. It is speculated thatlysosomes play a role in the process of ghost tangle formation and inbeta AP deposits leading to SP formation.
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| Emner |
