Metabolic Engineering : Principles and Methodologies
George. Stephanopoulos
Bok Engelsk 1998 · Electronic books.
| Utgitt | Burlington : : Elsevier Science, , 1998.
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| Omfang | 1 online resource (749 p.)
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| Opplysninger | Description based upon print version of record.. - Front Cover; Metabolic Engineering: Principles and Methodologies; Copyright Page; Contents; Preface; List of Symbols; Chapter 1. The Essence of Metabolic Engineering; 1.1 Importance of Metabolic Engineering; 1.2 General Overview of the Book; References; Chapter 2. Review of Cellular Metabolism; 2.1 An Overview of Cellular Metabolism; 2.2 Transport Processes; 2.3 Fueling Reactions; 2.4 Biosynthetic Reactions; 2.5 Polymerization; 2.6 Growth Energetics; References; Chapter 3. Comprehensive Models for Cellular Reactions; 3.1 Stoichiometry of Cellular Reactions; 3.2 Reaction Rates. - 11.1 Fundamentals of Metabolic Control Analysis11.2 Determination of Flux Control Coefficients; 11.3 MCA of Linear Pathways; 11.4 MCA of Branched Pathways; 11.5 Theory of Large Deviations; References; Chapter 12. Analysis of Structure of Metabolic Networks; 12.1 Control of Flux Distribution at a Single Branch Point; 12.2 Grouping of Reactions; 12.3 Case Study: Aromatic Amino Acid Biosynthetic Pathway; References; Chapter 13. Flux Analysis of Metabolic Networks; 13.1 Relationships among Group and Individual Control Coefficients (Bottom-Up Approach). - 13.2 Determination of Group Control Coefficients from Flux Measurements (Top-Down Approach). - 3.3 Dynamic Mass Balances3.4 Yield Coefficients and Linear Rate Equations; References; Chapter 4. Material Balances and Data Consistency; 4.1 The Black Box Model; 4.2 Elemental Balances; 4.3 Heat Balance; 4.4 Analysis of Overdetermined Systems-Identification of Gross Measurement Errors; References; Chapter 5. Regulation of Metabolic Pathways; 5.1 Regulation of Enzymatic Activity; 5.2 Regulation of Enzyme Concentration; 5.3 Global Control: Regulation at the Whole Cell Level; 5.4 Regulation of Metabolic Networks; References. - 8.3 Underdetermined Systems-Linear Programming8.4 Sensitivity Analysis; References; Chapter 9. Methods for the Experimental Determination of Metabolic Fluxes by Isotope Labeling; 9.1 Direct Flux Determination from Fractional Label Enrichment; 9.2 Applications Involving Complete Enumeration of Metabolite Isotopomers; 9.3 Carbon Metabolite Balances; References; Chapter 10. Applications of Metabolic Flux Analysis; 10.1 Amino Acid Production by Glutamic Acid Bacteria; 10.2 Metabolic Fluxes in Mammalian Cell Cultures; References; Chapter 11. Metabolic Control Analysis. - Chapter 6. Examples of Pathway Manipulations: Metabolic Engineering in Practice6.1 Enhancement of Product Yield and Productivity; 6.2 Extension of Substrate Range; 6.3 Extension of Product Spectrum and Novel Products; 6.4 Improvement of Cellular Properties; 6.5 Xenobiotic Degradation; References; Chapter 7. Metabolic Pathway Synthesis; 7.1 Metabolic Pathway Synthesis Algorithm; 7.2 Overview of the Algorithm; 7.3 A Case Study: Lysine Biosynthesis; 7.4 Discussion of the Algorithm; References; Chapter 8. Metabolic Flux Analysis; 8.1 Theory; 8.2 Overdetermined Systems. - Metabolic engineering is a new field with applications in the production of chemicals, fuels, materials, pharmaceuticals, and medicine at the genetic level. The field's novelty is in the synthesis of molecular biology techniques and the tools of mathematical analysis, which allow rational selection of targets for genetic modification through measurements and control of metabolic fluxes. The objective is to identify specific genetics or environmental manipulations that result in improvements in yield and productivities of biotechnological processes.Key features of the book are pathway integ
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| ISBN | 0126662606
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