Methods in bioengineering : nanoscale bioengineering and nanomedicine /
Kaushal Rege, Igor L. Medintz, editors.
Bok Engelsk 2009 · Electronic books.
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| Omfang | 1 online resource (364 p.)
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| Opplysninger | Description based upon print version of record.. - Methods in Bioengineering: Nanoscale Bioengineering and Nanomedicine; Contents; Preface; Chapter 1 Preparation and Characterization of Carbon Nanotube-Protein Conjugates; 1.1 Introduction; 1.2 Materials; 1.3 Methods; 1.3.1 Physical Adsorption of Proteins on Carbon Nanotubes; 1.3.2 Protein Assisted Solubilization of Carbon Nanotubes; 1.3.3 Covalent Attachment of Proteins onto Carbon Nanotubes; 1.4 Data Acquisition, Anticipated Results, and Interpretation of Data; 1.4.1 Characterization of Proteins Physically Adsorbed onto Carbon Nanotubes. - 1.4.2 Characterization of Protein-Solubilized Carbon Nanotubes1.4.3 Characterization of Covalently Attached Carbon Nanotube-Protein Conjugates; 1.5 Discussion and Commentary; 1.6 Applications Notes; 1.7 Summary Points; Acknowledgments; References; Chapter 2 Peptide-Nanoparticle Assemblies; 2.1 Introduction; 2.2 Materials; 2.3 Methods; 2.3.1 Coil-Coil Peptide Mediated NP Assembly; 2.3.2 Synthesis of Hybrid Structures Using Multifunctional Peptides; 2.4 Assembly Mediated by Metal Ion-Peptide Recognition; 2.5 Peptides as Antibody Epitopes for Nanoparticle Assembly. - 2.6 DATA Acquisition, Anticipated Results, and Interpretation2.7 Discussion and Commentary; 2.8 Application Notes; 2.9 Summary Points; Acknowledgments; References; Chapter 3 Nanoparticle-Enzyme Hybrids as Bioactive Materials; 3.1 Introduction; 3.2 Materials; 3.3 Methods; 3.3.1 Enzyme-Attached Polystyrene Nanoparticles; 3.3.2 Polyacrylamide Hydrogel Nanoparticles for Entrapment of Enzymes; 3.3.3 Magnetic Nanoparticles with Porous Silica Coating for Enzyme Attachment; 3.3.4 Enzyme Loading and Activity Assay; 3.4 Results; 3.4.1 Polystyrene-Enzyme Hybrid Nanoparticles. - 3.4.2 Polyacrylamide Hydrogel Nanoparticles with Entrapped Enzymes3.4.3 Magnetic Nanoparticles for Enzyme Attachment; 3.5 Discussion and Commentary; 3.6 Troubleshooting; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter 4 Self-Assembled QD-Protein Bioconjugates and Their Use in Fluorescence Resonance Energy Transfer; 4.1 Introduction; 4.2 Materials; 4.2.1 Reagents; 4.2.2 Equipment; 4.3 Methods; 4.3.1 Quantum Dot Synthesis; 4.3.2 Surface Ligand Exchange; 4.3.3 Biomolecule Conjugation; 4.3.4 Fluorescence Measurements; 4.4 Data Analysis and Interpretation. - 4.4.1 Calculating Donor-Acceptor Distances4.4.2 Calculating Reaction Rates of Surface-Bound Substrates; 4.5 Summary Points; 4.6 Conclusions; References; Annotated References; Chapter 5 Tracking Single Biomolecules in Live Cells Using Quantum Dot Nanoparticles; 5.1 Introduction; 5.2 Materials; 5.2.1 Reagents; 5.2.2 Imaging Equipment; 5.3 Methods; 5.3.1 Forming QD Bioconjugates; 5.3.2 Treating Cells with QD Bioconjugates; 5.4 Data Acquisition, Anticipated Results, and Interpretation; 5.4.1 Imaging QD-Bound Complexes in Cells; 5.4.2 Analysis of the Real-Time QD Dynamics. - 5.5 Discussion and Commentary. - This practical book is part of the new Artech House Methods in Bioengineering series - volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. This volume is focused on the materials involved with nanoscale bioengineering. Nanomaterials are quickly moving into the mainstream as a critical component of biological research. Filling a critical gap in the current literature, this new resource presents practical, step-by-step methods to help professionals synthesize, characterize, functionalize and apply the nanomaterial that is most suitable for handling a given nanoscale bioengineering problem. Written and presented by the best scientists and engineers in their respective fields, the authors offer a clear and detailed understanding of how to carry out a wide range of important methods in this area.
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| Emner | |
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| ISBN | 1-5231-4633-8. - 1-59693-411-5
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