High-Throughput Metabolomics : Methods and Protocols /


edited by Angelo D'Alessandro.
Bok Engelsk 2019 · Electronic books.
Medvirkende
Omfang
1 online resource (XV, 459 p. 83 illus., 63 illus. in color.)
Utgave
1st ed. 2019.
Opplysninger
Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells -- High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods -- High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research -- Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications -- CE-MS for Metabolomics: A Comparison with Other Techniques -- Overview of Lipid Mass Spectrometry and Lipidomics -- LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins -- Untargeted and Semi-Targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics -- HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle -- Quantification of D- and L-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry -- Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples -- Untargeted Metabolomics of Plant Leaf Tissues -- Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling -- Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry -- Insights into Dynamic Network States Using Metabolomic Data -- Analysis of Endothelial Fatty Acid Metabolism Using Tracer Metabolomics -- Using Stable Isotope Tracers for Metabolic Pathway Analysis -- Data Processing for GC-MS and LC-MS-Based Untargeted Metabolomics -- El-MAVEN: A Fast, Robust, and User-Friendly Mass Spectrometry Data Processing Engine for Metabolomics -- Pre-Analytic Considerations for Mass Spectrometry-Based Untargeted Metabolomics Data -- Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically-Encoded Biosensors -- Microplate Assays for Spectrophotometric Measurement of Mitochondrial Enzyme Activity -- Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy -- Metabolomic Applications in Radiation Biodosimetry -- Metabolomics Analyses to Investigate the Role of Diet and Physical Training -- Blood Biomarkers in Sports Medicine and Performance and the Future of Metabolomics -- Personalized Metabolomics.. - This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.
Emner
Sjanger
Dewey
ISBN
1-4939-9236-8

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