14-3-3 protein, neuron-specific enolase, and S-100 protein incerebrospinal fluid of patients with Creutzfeldt-Jakob disease
P. Beaudry
Bok Engelsk 1999
| Utgitt | 1999
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| Omfang | Side 40- 46
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| Opplysninger | We explored simultaneously 14-3-3 protein, neuron-specific enolase(NSE), and one astroglial protein, S-100, recently proposed asCreutzfeld-Jakob disease (CJD) markers, in the cerebrospinal fluid(CSF) of 129 patients with suspected CJD. Cutoff values for NSE and S-100 were established at 25 and 2.5 ng/ml, respectively The highestsensitivity was observed for S-100 (94.2%) followed by 14-3-3 (89.8%)and NSE (79.7%), while the highest specificity in CJD diagnosis wasobtained with 14-3-3 protein (100%) as compared with NSE (91.5%) andS-100 (85.4%). No influence of sex, genotype at codon 129 of theprion protein gene, time between sampling, and death or diseaseduration has been found. Based on 90 cases initially referred as'probable' or 'possible' CJD, with 14-3-3, NSE, or S-100 we couldcorrectly discriminate between 'CJD' or 'non-CJD' categories in 94.4,86.5, and 90% of the cases, respectively. When limited to 'possibleCJD' cases, diagnosis based on one of the three CSF proteins wasaccurate in 98, 90.7 and 87.3%, respectively. In view of the factthat the CSF 14-3-3 protein test alone has the highest specificityand good sensitivity, it appears that there is no additionaladvantage at the moment to include NSE and/or S-100 protein in theexploration of clinically suspected CJD cases.
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| Emner |
