Monomeric and polymeric forms of alpha-1 antichymotrypsin in serafrom patients with probable late onset Alzheimer's disease
F. Licastro
Bok Engelsk 1997
| Utgitt | 1997
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| Omfang | Side 337- 342
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| Opplysninger | Patients with probable late-onset Alzheimer's disease (1-AD) hadhigher levels of serum alpha(1)-antichymotrypsin (alpha(1)-ACT) thanthose found in patients with vascular dementia (VD) and healthyelderly controls, when assessed by a competitive enzyme-linked immuneassay. Serum alpha(1)-ACT was also characterized by SDS PAGEelectrophoresis, Western blot and computer-assisted optical densityreading (OD). Using a polyclonal affinity-purified antibody specificfor human alpha(1)-ACT, one band with the apparent MW of 60 andanother with 180 kD in sera from all subjects were clearlydetectable. OD of both alpha(1)-ACT bands from patients with 1-AD washigher than that from VD patients, the 180-kD form being 2.65 timeshigher than that observed from patients with VD. Serum levels ofother acute phase proteins from 1-AD were comparable to thoseobserved in VD patients. A slight but nonstatistical increment ofserum IL-6 was noted in patients with 1-AD. Serum alpha(1)-ACT waspurified from 3 of these 1-AD patients by a two-step affinitychromatography technique. After Western blot, purified alpha(1)-ACTshowed two or three different bands which immune-reacted with anantibody specific for alpha(1)-ACT. The apparent MWs were 60, 120 and180 kD. In human sera the serpin was present mainly in a monomericform; but it could also form SDS stable dimers and trimers. Bothmonomeric and SDS stable polymeric forms of alpha(1)-ACT appeared tobe increased in sera from patients with 1-AD.
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| Emner |
