Protein-protein interactions : methods and applications /
edited by Cheryl L. Meyerkord, Haian Fu
Bok · Engelsk · 2015
| Omfang | XV, 620 s. : : ill. ;
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| Utgave | 2nd edition.
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| Opplysninger | Structural basis of protein-protein interactions -- Quantitative analysis of protein-protein interactions -- Protein-protein interaction databases -- Computational prediction of protein-protein interactions. -- Structure-based computational approaches for small-molecule modulation of protein-protein interactions -- Targeting protein-protein interactions for drug discovery -- Studying protein-protein interactions using surface plasmon resonance -- Resonant waveguide grating for monitoring bimolecular interactions -- Quartz microbalance technology for probing biomolecular interactions -- Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry -- Characterization of protein-protein interactions by isothermal titration calorimetry -- Sedimentation equilibrium studies -- Detecting protein-protein interactions by gel filtration chromatography -- Using light scattering to determine the stoichiometry of protein complexes-- Circular dichroism (CD) analyses of protein-protein interactions -- Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy -- Quantitative protein analysis by mass spectrometry -- Using peptide arrays created by the SPOT method for defining protein-protein interactions -- Fluorescence polarization assay to quantify protein-protein interactions -- Fèorster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions -- Utilizing ELISA to monitor protein-protein interactions -- Glutathione-S-transferase (GST)-fusion based assays for studying protein-protein interactions -- Hexahistidine (6xHis) fusion-based assays for protein-protein interactions -- Studying protein-protein interactions via blot overlay/Far Western blot -- Co-immunoprecipitation from transfected cells -- In vivo protein cross-linking -- Identification of protein-protein interactions by standard Gal4p-based yeast two-hybrid screening-- Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae -- MAPPIT, a mammalian two-hybrid method for in-cell detection of protein-protein interactions -- Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells -- Mapping biochemical networks with protein fragment complementation assays -- Detection of protein-protein interaction using bimolecular fluorescence complementation assay -- Split-luciferase complementation assay to detect channel-protein interactions in live cells -- Confocal microscopy for intracellular co-localization of proteins -- Fluorescence polarization assay to quantify protein-protein interactions in an HTS format -- Estrogen receptor alpha/co-activator interaction assay -- TR-FRET -- High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions -- Discovery of inhibitors of the MDM2-p53 protein-protein interaction-- Biophysical methods for identifying fragment-based inhibitors of protein-protein interactions.
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| Emner | |
| Dewey | |
| ISBN | 1493924249. - 9781493924240
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