A-Z of quantitative PCR
edited by Stephen A. Bustin
Bok · Engelsk · 2004
| Annen tittel | |
|---|---|
| Utgitt | La Jolla, CA : : International University Line, , 2004
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| Omfang | xxix, 882 s. : : ill. (noen farg.) ;
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| Opplysninger | Quantification of nucleic acids by PCR -- Real-time RT-PCR: what lies beneath the surface -- Quantification strategies in real-time PCR -- Good laboratory practice! -- Template handling, preparation and quantification -- Chemistries -- Primers and probes -- Instrumentation -- Basic RT-PCR considerations -- The PCR step -- Data anaysis and interpretation -- The qPCR does not work? -- Getting started - the basics of setting up a qPCR assay -- Use of standardized mixtures of internal standards in quantitative RT-PCR to ensure quality control and develop a standardized gene expression database -- Standardization of qPCR and qRT-PCR assays -- Extraction of total RNA from formalin-fixed paraffin-embedded tissue -- Cells-to-cDNA II: RT-PCR without RNA isolation -- Optimization of single and multiplex real-time PCR -- Evaluation of basic fibroblast growth factor mRNA levels in breast cancer -- Detection of "tissue-specific" mRNA in the blood and lymph nodes of patients without colorectal cancer -- Optimized real-time RT-PCR for quantitative measurements of DNA and RNA in single embryos and their blastomeres -- Single cell global RT and quantitative real-time PCR -- Single nucleotide polymorphism detection with fluorescent MGB eclipse probe systems -- Genotyping using MGB-hydrolysis probes -- Scorpions primers for real-time genotyping and quantitative genotyping of pooled DNA -- Simultaneous detection and sub-typing of human papillomavirus in the cervix using real-time quantitative PCR
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| Emner | |
| Dewey | |
| ISBN | 0-9636817-8-8. - 978-0-9636817-8-2
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