Monoclonal Antibody and Immunosensor Technology : The production and application of rodent and human monoclonal antibodies


A.M. Campbell
Bok Engelsk 1991 · Electronic books.
Annen tittel
Utgitt
Burlington : : Elsevier Science, , 1991.
Omfang
1 online resource (455 p.)
Opplysninger
Description based upon print version of record.. - Cover; TOCContents; Preface; Acknowledgements; Abbrevations; CHChapter 1. General properties and applications of monoclonal antibodies; 1.1. History of Mab technology; 1.2. Introduction to Mab technology; 1.3. Specificity/affinity - the contribution of monoclonal antibody; 1.4. Specificity/affinity - the contribution of antigen; 1.5. Specificity/affinity - the contribution of the assay system; 1.6. Pooling of Mabs; 1.7. The standard nature of Mabs; 1.8. Yield and purity; 1.9. Human monoclonal antibodies (HuMabs); 1.10. Chimaeric bifunctional monoclonal antibodies. - 1.11. Applications of Mabs - Diagnostic1.12. Therapeutic applications; 1.13. Mabs in vaccine development strategies; 1.14. Mabs as catalysts (abzymes); 1.15. Mabs in purification; 1.16. Mabs in basic immunological research; 1.17. Patent considerations for Mabs; 1.18. Buying a Mab; CHChapter 2. Assay techniques; 2.1. General assay requirements; 2.2. Theoretical considerations; 2.3. Practical screening in the lab; 2.4. Antibody sampling; 2.5. Types of assay; 2.6. Solid-phase assays; 2.7. Protocols for solid-phase assays; 2.8. Screening for cellular antigens by FACS. - 2.9. Screening for cellular antigens by immunocytochemical methods2.10. Screening for catalytic antibodies; 2.11. Screening recombinant libraries; CHChapter 3. Selection of animals, tissues and cell lines; 3.1. Animals in which hybridomas may be generated; 3.2. Whether to choose the rat or mouse system; 3.3. Selective drug markers; 3.4. The mouse system; 3.5. The rat system; 3.6. The human system; 3.7. T cell lines; 3.8. Tissues used as sources of B cells; CHChapter 4. Immunisation; 4.1. How the natural immune response operates; 4.2. Is it necessary to immunise?. - 4.19. Immunisation of human subjectsCHChapter 5. Cell culture requirements for hybridomas; 5.1. Introduction; 5.2. Basic cell culture requirements; 5.3. Basic cell culture techniques; 5.4. Contamination; 5.5. Feeder cells; CHChapter 6. Fusion procedures; 6.1. The use of polyethylene glycol; 6.2. The components of HAT medium; 6.3. Selection of hybridomas by fluorescent activated cell sorting (FACS); 6.4. Preparation of stock solutions; 6.5. Fusion frequencies and plating densities; 6.6. Fusion protocols for mouse experiments; 6.7. Electrofusion. - 4.3. Is it necessary to know precisely what the antigen is?4.4. Purity of antigen; 4.5. The use of previously determined schedules; 4.6. Strains of rodent; 4.7. Age and sex of rodent; 4.8. Tolerance, overimmunisation and underimmunisation; 4.9. Adjuvants; 4.10. In vitro immunisation; 4.11. Production of specific antibody isotypes; 4.12. Routes of immunisation; 4.13. Schedules; 4.14. Specific examples - proteins; 4.15. Specific examples - carbohydrates; 4.16. Specific examples - lipids and nucleic acids; 4.17. Specific examples - haptens; 4.18. Specific examples - whole cells and organisms. - CHChapter 7. Alternative strategies for the immortalisation of antibody producing cells. - This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene tr
Emner
Sjanger
Dewey
ISBN
0444814124. - 0444814132

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