Tetraethylammonium-induced calcium concentration changes in skin fibroblasts from patients with alzheimer disease
S.S. Matsuyama
Bok Engelsk
| Utgitt | SS Matsuyama W Los Angeles Vet Affairs Med Ctr
|
|---|---|
| Opplysninger | Potassium (Kc) channel dysfunction in fibroblasts was recently proposed as a potential diagnostic marker for Alzheimer disease (AD), We utilized a microspectrofluorometric method with Fura-2AM to measure intracellular free calcium ([Ca2+](i)) following depolarization with the K+ channel blocker tetraethylammonium (TEA) in seven AD and seven control fibroblast cultures, Contrary to our expectation, 43% of the AD and 36% of the control fibroblast plated coverglasses responded with an increase in [Ca2+](i) on addition of 100 mM TEA. The data suggest that the TEA- elicited [Ca2+](i) response is not a useful AD screening test.
|
| Emner | |
| ISSN |
